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rabbit polyclonal anti cyck  (Abcam)

 
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    Structured Review

    Abcam rabbit polyclonal anti cyck
    (A) The indicated CD8-ICL4 fusion proteins were expressed with HIV-1 expressing Nef with a dileucine substitution (Nef LL/AA). Proteins were co-immunoprecipitated with a <t>polyclonal</t> anti-Nef antibody and detected by WB using the indicated antibodies. (B) CoIP samples 1, 2, and 3 in (A) were analyzed by MS after trypsin digestion. Nef, CD8, and ICL4 peptides detected from these samples are underlined. Three ICL4 peptides are numbered (1, 2, 3). (C) Levels of Nef, CD8, and ICL4 peptides detected by MS in (B) were quantified by label-free quantification (LFQ) and their intensity is presented as relative values, with the value of WT CD8-ICL4 set to 100%. Nef and CD8 were quantified by all detectable peptides and ICL4 was quantified by peptide-1 (SSSDALQGR). The error bars indicate SEM calculated from three independent experiments. Statistical analysis: *p < 0.05, **p < 0.01; ns, p > 0.05. (D) A proposed model of how S360 phosphorylation promotes SERINC5 downregulation.
    Rabbit Polyclonal Anti Cyck, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 9192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti cyck/product/Abcam
    Average 98 stars, based on 9192 article reviews
    rabbit polyclonal anti cyck - by Bioz Stars, 2026-03
    98/100 stars

    Images

    1) Product Images from "HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity"

    Article Title: HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity

    Journal: Cell reports

    doi: 10.1016/j.celrep.2021.109514

    (A) The indicated CD8-ICL4 fusion proteins were expressed with HIV-1 expressing Nef with a dileucine substitution (Nef LL/AA). Proteins were co-immunoprecipitated with a polyclonal anti-Nef antibody and detected by WB using the indicated antibodies. (B) CoIP samples 1, 2, and 3 in (A) were analyzed by MS after trypsin digestion. Nef, CD8, and ICL4 peptides detected from these samples are underlined. Three ICL4 peptides are numbered (1, 2, 3). (C) Levels of Nef, CD8, and ICL4 peptides detected by MS in (B) were quantified by label-free quantification (LFQ) and their intensity is presented as relative values, with the value of WT CD8-ICL4 set to 100%. Nef and CD8 were quantified by all detectable peptides and ICL4 was quantified by peptide-1 (SSSDALQGR). The error bars indicate SEM calculated from three independent experiments. Statistical analysis: *p < 0.05, **p < 0.01; ns, p > 0.05. (D) A proposed model of how S360 phosphorylation promotes SERINC5 downregulation.
    Figure Legend Snippet: (A) The indicated CD8-ICL4 fusion proteins were expressed with HIV-1 expressing Nef with a dileucine substitution (Nef LL/AA). Proteins were co-immunoprecipitated with a polyclonal anti-Nef antibody and detected by WB using the indicated antibodies. (B) CoIP samples 1, 2, and 3 in (A) were analyzed by MS after trypsin digestion. Nef, CD8, and ICL4 peptides detected from these samples are underlined. Three ICL4 peptides are numbered (1, 2, 3). (C) Levels of Nef, CD8, and ICL4 peptides detected by MS in (B) were quantified by label-free quantification (LFQ) and their intensity is presented as relative values, with the value of WT CD8-ICL4 set to 100%. Nef and CD8 were quantified by all detectable peptides and ICL4 was quantified by peptide-1 (SSSDALQGR). The error bars indicate SEM calculated from three independent experiments. Statistical analysis: *p < 0.05, **p < 0.01; ns, p > 0.05. (D) A proposed model of how S360 phosphorylation promotes SERINC5 downregulation.

    Techniques Used: Expressing, Immunoprecipitation

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Protease Inhibitor, Mutagenesis, Clone Assay, Luciferase, Staining, Knock-Out, Marker, Software



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    rabbit polyclonal anti cyck  (Abcam)
    98
    Abcam rabbit polyclonal anti cyck
    (A) The indicated CD8-ICL4 fusion proteins were expressed with HIV-1 expressing Nef with a dileucine substitution (Nef LL/AA). Proteins were co-immunoprecipitated with a <t>polyclonal</t> anti-Nef antibody and detected by WB using the indicated antibodies. (B) CoIP samples 1, 2, and 3 in (A) were analyzed by MS after trypsin digestion. Nef, CD8, and ICL4 peptides detected from these samples are underlined. Three ICL4 peptides are numbered (1, 2, 3). (C) Levels of Nef, CD8, and ICL4 peptides detected by MS in (B) were quantified by label-free quantification (LFQ) and their intensity is presented as relative values, with the value of WT CD8-ICL4 set to 100%. Nef and CD8 were quantified by all detectable peptides and ICL4 was quantified by peptide-1 (SSSDALQGR). The error bars indicate SEM calculated from three independent experiments. Statistical analysis: *p < 0.05, **p < 0.01; ns, p > 0.05. (D) A proposed model of how S360 phosphorylation promotes SERINC5 downregulation.
    Rabbit Polyclonal Anti Cyck, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti cyck/product/Abcam
    Average 98 stars, based on 1 article reviews
    rabbit polyclonal anti cyck - by Bioz Stars, 2026-03
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) The indicated CD8-ICL4 fusion proteins were expressed with HIV-1 expressing Nef with a dileucine substitution (Nef LL/AA). Proteins were co-immunoprecipitated with a polyclonal anti-Nef antibody and detected by WB using the indicated antibodies. (B) CoIP samples 1, 2, and 3 in (A) were analyzed by MS after trypsin digestion. Nef, CD8, and ICL4 peptides detected from these samples are underlined. Three ICL4 peptides are numbered (1, 2, 3). (C) Levels of Nef, CD8, and ICL4 peptides detected by MS in (B) were quantified by label-free quantification (LFQ) and their intensity is presented as relative values, with the value of WT CD8-ICL4 set to 100%. Nef and CD8 were quantified by all detectable peptides and ICL4 was quantified by peptide-1 (SSSDALQGR). The error bars indicate SEM calculated from three independent experiments. Statistical analysis: *p < 0.05, **p < 0.01; ns, p > 0.05. (D) A proposed model of how S360 phosphorylation promotes SERINC5 downregulation.

    Journal: Cell reports

    Article Title: HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity

    doi: 10.1016/j.celrep.2021.109514

    Figure Lengend Snippet: (A) The indicated CD8-ICL4 fusion proteins were expressed with HIV-1 expressing Nef with a dileucine substitution (Nef LL/AA). Proteins were co-immunoprecipitated with a polyclonal anti-Nef antibody and detected by WB using the indicated antibodies. (B) CoIP samples 1, 2, and 3 in (A) were analyzed by MS after trypsin digestion. Nef, CD8, and ICL4 peptides detected from these samples are underlined. Three ICL4 peptides are numbered (1, 2, 3). (C) Levels of Nef, CD8, and ICL4 peptides detected by MS in (B) were quantified by label-free quantification (LFQ) and their intensity is presented as relative values, with the value of WT CD8-ICL4 set to 100%. Nef and CD8 were quantified by all detectable peptides and ICL4 was quantified by peptide-1 (SSSDALQGR). The error bars indicate SEM calculated from three independent experiments. Statistical analysis: *p < 0.05, **p < 0.01; ns, p > 0.05. (D) A proposed model of how S360 phosphorylation promotes SERINC5 downregulation.

    Article Snippet: Horseradish peroxidase (HRP)-conjugated mouse monoclonal anti-FLAG, anti-HA antibodies, and anti-actin were purchased from Sigma; HRP-conjugated mouse monoclonal anti-GAPDH was purchased from Proteintech; rabbit polyclonal anti-CrkRS (CDK12) and anti-CDC2L5 (CDK13) were purchased from Novus; rabbit polyclonal anti-CycK was purchased from Abcam; HRP-conjugated anti-human, -rabbit, and -mouse immunoglobulin G secondary antibodies were purchased from Thermo Fisher.

    Techniques: Expressing, Immunoprecipitation

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity

    doi: 10.1016/j.celrep.2021.109514

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Horseradish peroxidase (HRP)-conjugated mouse monoclonal anti-FLAG, anti-HA antibodies, and anti-actin were purchased from Sigma; HRP-conjugated mouse monoclonal anti-GAPDH was purchased from Proteintech; rabbit polyclonal anti-CrkRS (CDK12) and anti-CDC2L5 (CDK13) were purchased from Novus; rabbit polyclonal anti-CycK was purchased from Abcam; HRP-conjugated anti-human, -rabbit, and -mouse immunoglobulin G secondary antibodies were purchased from Thermo Fisher.

    Techniques: Recombinant, Protease Inhibitor, Mutagenesis, Clone Assay, Luciferase, Staining, Knock-Out, Marker, Software